Skip to main content
Department of Pediatrics Department of Pediatrics
  1. HOME
  2. Research
  3. Faculty Labs
  4. Haddad Lab
  5. Publications

Publications

Selected Publications:

  1. Stobdan T, Jain PP, Xiong M, Bafna V, Yuan J, Haddad GG. Heterozygous Tropomodulin 3 mice have improved lung vascularization after chronic hypoxia. Human Molecular Genetics. 2021 Oct 28;ddab291. doi: 10.1093/hmg/ddab291.
  2. Zhou D, Xue J, Miyamoto Y, Poulsen O, Eckmann L, Haddad GG. Microbiota Modulates Cardiac Transcriptional Responses to Intermittent Hypoxia and Hypercapnia. Front Physiol. 2021 Jun 25; 12:680275. doi: 10.3389/fphys.2021.680275. PMID: 34248668; PMCID: PMC8267877.
  3. Lopez-Ramirez MA, Lai CC, Soliman SI, Hale P, Pham A, Estrada EJ, McCurdy S, Girard R, Verma R, Moore T, Lightle R, Hobson N, Shenkar R, Poulsen O, Haddad GG, Daneman R, Gongol B, Sun H, Lagarrigue F, Awad IA, Ginsberg MH. Astrocytes propel neurovascular dysfunction during cerebral cavernous malformation lesion formation. J Clin Invest. 2021 Jul 1;131(13):e139570. doi: 10.1172/JCI139570. PMID: 34043589.
  4. Allaband C, Lingaraju A, Martino C, Russell B, Tripathi A, Poulsen O, Dantas Machado AC, Zhou D, Xue J, Elijah E, Malhotra A, Dorrestein PC, Knight R, Haddad GG, Zarrinpar A. Intermittent Hypoxia and Hypercapnia Alter Diurnal Rhythms of Luminal Gut Microbiome and Metabolome. mSystems. 2021 Jun 29;e0011621. doi: 10.1128/mSystems.00116-21. PMID: 34184915
  5. Negraes PD, Trujillo CA, Yu NK, Wu W, Yao H,
Liang N, Lautz JD, Kwok K, McClatchy D, Diedrich J, Bartolome SM, Romero S, Turori C, Brittney VH , Herai RH, Smith SE, Haddad GG, Yates JR, Muotri AR. Altered network and rescue of human neurons derived from individuals with early-onset genetic epilepsy. Molecular Psychiatry. 2021 Apr 22. doi: 10.1038/s41380-021-01104-2. PMID: 33888873.In press.
  6. Xue J, Allaband C, Zhou D, Poulsen O, Jiang L, Tripathi A, Dorrestein PC, Knight R, Zarrinpar A, Haddad GG. Influence of Intermittent Hypoxia/Hypercapnia on Atherosclerosis, Gut Microbiome and Metabolome. Frontiers in Physiology – Integrative Physiology. 2021 Apr 8;12:663950. doi: 10.3389/ fphys.2021.663950. PMID: 33897472.
  7. Iranmehr A, Stobdan T, Zhou D, Zhao H, Kryazhimskiy S, Bafna V, Haddad GG. Multiple mechanisms drive genomic adaptation to extreme O2 levels over two decades in Drosophila melanogaster. Nature Communication. 2021 Feb 12;12(1):997. doi: 10.1038/s41467-021-21281-6. PMID: 33579965.
  8. Zhao HW, Lin J, Sieck G, Haddad GG. Neuroprotective Role of Akt in Hypoxia Adaptation in Andeans. Frontiers in Neuroscience – Neurodegeneration. 2021 Jan 15;14:607711. doi: 10.3389/ fnins.2020.607711. eCollection 2020. PMID: 33519361.

Mouse Astrocutes. Green: GFAP, Blue: DAPI

  1. Azad P, Villafuerte FC, Bermudez D, Patel G, Haddad GG. Protective Role of Estrogen Against Excessive Erythrocytosis In Monge's Disease. Experimental and Molecular Medicine. 2021;53:125-135. PMID: 33473144
  2. Zhou D, Stobdan T, Visk D, Xue J, Haddad GG. Genetic interactions regulate hypoxia tolerance conferred by activating Notch in excitatory amino acid transporter 1-positive glial cells in Drosophila melanogaster. G3: Genes Genomes Genetics (Bethesda). 2021 Feb 9:11(2):jkab038. doi: 10.1093/g3journal/jkab038. PMID: 33576765.
  3. Wu W, Yao H, Dwivedi I, Negraes PD, Zhao HW, Wang J, Trujillo CA, Muotri AR, Haddad GG. Methadone suppresses neuronal function and maturation in human cortical organoids. Frontiers in Neuroscience - Neuropharmacology. 2020 Nov 23;14:593248. doi: 10.3389/fnins.2020.593248. eCollection 2020. PMID: 33328864.
  4. Yao H, Wu W, Cerf I, Zhao HW, Wang J, Negraes PD, Muotri, AR, Haddad GG. Methadone interrupts neural growth and function in human cortical organoids. Stem Cell Research. 2020 Dec;49:102065. doi: 10.1016/j.scr.2020.102065. Epub 2020 Oct 27.
  5. Engelhart DC, Azad P, Ali S, Granados JC, Haddad GG, Nigam SK. Drosophila SLC22 Orthologs Related to OATs, OCTs, and OCTNs Regulate Development and Responsiveness to Oxidative Stress. Int J Mol Sci. 2020 Mar 15;21(6):2002. doi: 10.3390/ijms21062002.
  6. Quinn RA, Melnik AV, Vrbanac A, Fu T, Patras KA, Christy MP, Bodai Z, Belda-Ferre P, Tripathi A, Chung LK, Downes M, Welch RD, Quinn M, Humphrey G, Panitchpakdi M, Weldon KC, Aksenov A, da Silva R, Avila-Pacheco J, Clish C, Bae S, Mallick H, Franzosa EA, Lloyd-Price J, Bussell R, Thron T, Nelson AT, Wang M, Leszczynski E, Vargas F, Gauglitz JM, Meehan MJ, Gentry E, Arthur TD, Komor AC, Poulsen O, Boland BS, Chang JT, Sandborn WJ, Lim M, Garg N, Lumeng JC, Xavier RJ, Kazmierczak BI, Jain R, Egan M, Rhee KE, Ferguson D, Raffatellu M, Vlamakis H, Haddad GG, Siegel D, Huttenhower C, Mazmanian SK, Evans RM, Nizet V, Knight R, Dorrestein PC. Global chemical effects of the microbiome include new bile-acid conjugations. Nature. 2020 Mar;579(7797):123-129. doi: 10.1038/s41586-020-2047-9.
  7. Bermudez D, Azad P, Figueroa-Mujíca R, Vizcardo-Galindo G, Corante N, Guerra-Giraldez C, Haddad GG, Villafuerte FC. Increased hypoxic proliferative response and gene expression in erythroid progenitor cells of Andean highlanders with chronic mountain sickness. Am J Physiol Regul Integr Comp Physiol. 2020 Jan 1;318(1):R49-R56. doi: 10.1152/ajpregu.00250.2019.
  8. Imamura T, Xue J, Poulsen O, Zhou D, Karin M, Haddad GG. Intermittent hypoxia and hypercapnia induces inhibitor of nuclear factor-κB kinase subunit β-dependent atherosclerosis in pulmonary arteries. Am J Physiol Regul Integr Comp Physiol. 2019 Dec 1;317(6):R763-R769. doi: 10.1152/ ajpregu.00056.2019.
human cerebral

Human cerebral organoids derived from iPSCs

  1. Trujillo CA, Gao R, Negraes PD, Gu J, Buchanan J, Preissl S, Wang A, Wu W, Haddad GG, Chaim IA, Domissy A, Vandenberghe M, Devor A, Yeo GW, Voytek B, Muotri AR.Trujillo CA, et al. Complex Oscillatory Waves Emerging from Cortical Organoids Model Early Human Brain Network Development. Cell Stem Cell. 2019 Oct 3;25(4):558-569.e7. doi: 10.1016/j.stem.2019.08.002. Epub 2019 Aug 29.
  2. Tripathi A, Xu Z, Jin Xue, Poulsen O, González A, Humphrey G, Meehan M, Melnik A, Ackermann G, Zhou D, Malhotra A, Haddad GG, Dorrestein P, and Knight R. Intermittent hypoxia and hypercapnia reproducibly change the gut microbiome and metabolome across rodent model systems. mSystems. 2019 Apr 30;4(2):e00058-19. doi: 10.1128/mSystems.00058-19.
  3. Stobdan T and Haddad GG. Commentary: Novel Insight into the Genetic Basis of High Altitude Pulmonary Hypertension in Kyrgyz Highlanders. J Lung Health Dis. 2019; 3(2): 29-30.
  4. Stobdan T, Sahoo D, Azad P, Hartley I, Heinrichsen E, Zhou D, Haddad GG. High fat diet induces sex-specific differential gene expression in Drosophila melanogaster. PLoS One. 2019 Mar 12;14(3):e0213474. doi:10.1371/journal.pone.0213474. eCollection 2019. PubMed PMID: 30861021; PubMedCentral PMCID: PMC6413938.
  5. Ghosh SG, Becker K, Huang H, Dixon-Salazar T, Chai G, Salpietro V, Al-Gazali L, Waisfisz Q, Wang H, Vaux KK, Stanley V, Manole A, Akpulat U, Weiss MM, Efthymiou S, Hanna MG, Minetti C, Striano P, Pisciotta L, De Grandis E, Altmüller J, Nürnberg P, Thiele H, Yis U, Okur TD, Polat AI, Amiri N, Doosti M, Karimani EG, Toosi MB, Haddad GG, Karakaya M, Wirth B, van Hagen JM, Wolf NI, Maroofian R, Houlden H, Cirak S, Gleeson JG. Biallelic Mutations in ADPRHL2, Encoding ADP- Ribosylhydrolase 3, Lead to a DegenerativePediatric Stress-Induced Epileptic Ataxia Syndrome. Am J Hum Genet. 2018 Nov 1;103(5):826. doi: 10.1016/j.ajhg.2018.10.002. PMID: 30388405
  6. Iranmehr A, Stobdan T, Zhou D, Poulsen O, Strohl KP, Aldashev A, Telenti A, Wong EHM, Kirkness EF, Venter JC, Bafna V, Haddad GG. Novel insight into the genetic basis of high-altitude pulmonary hypertension in Kyrgyz highlanders. Eur J Hum Genet. 2018 Sep 25. doi: 10.1038/s41431-018-0270-8. [Epub ahead of print] PMID: 30254217
  7. Stobdan T, Zhou D, Williams AT, Cabrales P, Haddad GG. Cardiac-specific knockout and pharmacological inhibition of Endothelin receptor type B lead to cardiac resistance to extreme hypoxia. J Mol Med (Berl). 2018 Sep;96(9):975-982. doi: 10.1007/s00109-018-1673-2. Epub 2018 Aug 1. PMID: 30069745
  8. Xue J, Zhou D, Poulsen O, Hartley I, Imamura T, Xie EX, Haddad GG. Exploring miRNA-mRNA regulatory network in cardiac pathology in Na+/H+ exchanger isoform 1 transgenic mice. Physiol Genomics. 2018 Oct 1;50(10):846-861. doi: 10.1152/physiolgenomics.00048.2018. Epub 2018 Jul 20. PMID: 30029588
astrocytes

GFP labeled mouse astrocytes culture in a dish

  1. Tripathi A, Melnik AV, Xue J, Poulsen O, Meehan MJ, Humphrey G, Jiang L, Ackermann G, McDonald D, Zhou D, Knight R, Dorrestein PC, Haddad GG. Intermittent Hypoxia and Hypercapnia, a Hallmark of Obstructive Sleep Apnea, Alters the Gut Microbiome and Metabolome. mSystems. 2018 Jun 5;3(3). pii: e00020-18. doi: 10.1128/mSystems.00020-18. eCollection 2018 May-Jun. PMID: 29896566
  2. Yao H, Zhao H, Wang J, Haddad GG. Intracellular pH Regulation in iPSCs-derived Astrocytes from Subjects with Chronic Mountain Sickness. Neuroscience. 2018 Apr 1;375:25-33. Doi: 10.1016/ j.neuroscience.2018.02.008. Epub 2018 Feb 10. PMID: 29438800
  3. Wu W, Yao H, Zhao HW, Wang J, Haddad GG. . Down-regulation of Inwardly Rectifying K+ Currents in Astrocytes Derived from Patients with Monge's Disease. Neuroscience. 2018 Jan 17. pii: S0306-4522(18)30032-0. doi: 10.1016/j.Neuroscience
  4. Zhao H, Perkins G, Yao H, Callacondo D, Appenzeller O, Ellisman M, La Spada A.R., Haddad GG Mitochondrial dysfunction in iPSC-derived neurons of subjects with chronic mountain sickness. J Appl Physiol. 2017 Dec 21.
  5. Azad P, Stobdan T, Zhou D, Hartley I, Akbari A, Bafna V, Haddad GG. High altitude adaptation in humans: from Genomics to Integrative Physiology. J Mol Med. 2017 Dec;95(12):1269-1282. doi: 10.1007/s00109-017-1584-7
  6. Stobdan T, Akbari A, Azad P, Zhou D, Poulsen O, Appenzeller O, Gonzales GF, Telenti A, Wong EHM, Shubham S, Kirkness EF, Venter JC, Bafna V, Haddad GG. New insights into the genetic basis of Monge's disease and adaptation to high-altitude. Molecular Biology & Evolution. 2017 Dec 11;34(12):3154-3168
  7. Gan Z, Powell FL, Zambon AC, Buchholz KS, Fu Z, Ocorr K, Bodmer R, Moya EA, Stowe JC, Haddad GG, McCulloch AD. Transcriptomic analysis identifies a role of PI3K-Akt signalling in the responses of skeletal muscle to acute hypoxia in vivo. J Physiol. 2017 Sep 1;595(17):5797-5813. doi: 10.1113/JP274556
  8. Xue J, Zhou D, Poulsen O, Imamura I, Hsiao YH, Smith T, Malhotra A, Dorrestein P, Knight R, Haddad GG (2017). "Intermittent Hypoxia and Hypercapnia Accelerate Atherosclerosis, Partially via Trimethylamine-Oxide". Am J of Respir Cell Mol Biol. doi: 10.1165/rcmb.2017-0086OC.

3D corticoids express neuronal markers

  1. Azad P, Zhao HW, Cabrales PJ, Ronen R, Zhou D, Poulsen O, Appenzeller O, Hsiao YH, Bafna V and Haddad GG (2016). Senp1 drives hypoxia-induced polycythemia via GATA1 and Bcl-xL in subjects with Monge's disease. J Exp Med. 213(12):2729-2744.
  2. Yao H, Azad P, Zhao HW, Wang J, Poulsen O, Freitas BC, Muotri AR, Haddad GG., The Na+/HCO3- co-transporter is protective during ischemia in astrocytes. Neuroscience. 2016 Oct 4. pii: S0306-4522(16)30507-3. doi: 10.1016/j.neuroscience.2016.09.050. [Epub ahead of print] PMID: 27717805
  3. Imamura T, Poulsen O, Haddad GG. Intermittent hypoxia induces murine macrophage foam cell formation by IKK- β-dependent NF-kB pathway activation. J Appl Physiol (1985). 2016 Sep 1;121(3):670-7. doi: 10.1152/japplphysiol.00307.2016. PMID:27471237
  4. Aggarwal DD, Rashkovetsky E, Michalak P, Cohen I, Ronin Y, Zhou D, Haddad GG, Korol AB. Experimental evolution of recombination and crossover interference in Drosophila caused by directional selection for stress-related traits. BMC Biol. 2015 Nov 27;13(1):101. PMID: 26614097
  5. Zarndt R, Piloto S, Powell FL, Haddad GG, Bodmer R, Ocorr K. Cardiac responses to hypoxia and reoxygenation in Drosophila. Am J Physiol Regul Integr Comp Physiol. 2015 Sep 16:ajpregu.00164.2015. PMID: 26377557
  6. Jha AR, Zhou D, Brown CD, Kreitman M, Haddad GG, White KP. Shared genetic signals of hypoxia adaptation in Drosophila and in high-altitude human populations. Mol Biol Evol. 2015 Nov 17; 33(2):501-517. PMID: 26576852
  7. Stobdan T, Zhou D, Ao-Ieong E, Ortiz D, Ronen R, Hartley I, Gan Z, McCulloch A, Bafna V, Cabrales P, Haddad GG. Endothelin Receptor B, a candidate gene from human studies at high altitude, improves cardiac tolerance to hypoxia in genetically engineered heterozygote mice. Proc Natl Acad Sci USA. 2015 Aug. 3, PMID: 26240367.
  8. Manchenkov T, Pasillas MP, Haddad GG, Imam FB. Novel Genes Critical for Hypoxic Preconditioning in Zebrafish Are Regulators of Insulin and Glucose Metabolism. G3 (Bethesda). 2015 Apr 3;5(6):1107-16.  PMID: 25840431.

Human astrocytes culture from an astrosphere derived from iPSC

  1. Zhao HW, Gu XQ, Chailangkarn T, Perkins G, Callacondo D, Appenzeller O, Poulsen O, Zhou D, Muotri AR, Haddad GG. Altered iPSC-derived neurons' sodium channel properties in subjects with Monge's disease. Neuroscience. 2015 Mar 12;288:187-99. PMID: 25559931.
  2. Smith KA1, Voiriot G, Tang H, Fraidenburg DR, Song S, Yamamura H, Yamamura A, Guo Q, Wan J, Pohl NM, Tauseef M, Bodmer R, Ocorr K, Thistlethwaite PA, Haddad GG, Powell FL, Makino A, Mehta D, Yuan JX. Notch Activation of Ca+ Signaling Mediates Hypoxic Pulmonary Vasoconstriction and Pulmonary Hypertension. Am J Respir Cell Mol Biol. 2015 Jan 8. PMID: 2556985.
  3. Pamenter ME, Haddad GG. High-throughput cell death assays. Methods Mol Biol. 2015; 1254:153-63. PMID: 25431064.
  4. Griesi-Oliveira K, Acab A, Gupta AR, Sunaga DY, Chailangkarn T, Nicol X, Nunez Y, Walker MF, Murdoch JD, Sanders SJ, Fernandez TV, Ji W, Lifton RP, Vadasz E, Dietrich A, Pradhan D, Song H, Ming GL, Gu X, Haddad GG, Marchetto MC, Spitzer N, Passos-Bueno MR, State MW, Muotri AR. Modeling non-syndromic autism and the impact of TRPC6 disruption in human neurons. Mol Psychiatry. 2014. PMID: 25385366. 
  5. Huang H, Lu-Bo Y, Haddad GG. A Drosophila ABC transporter regulates ROS and lifespan. PLoS Genetics. 2014 Dec;10(12):e1004844. PMID: PMID:25474322.
  6. Gersten M, Zhou D, Azad P, Haddad GG, Subramaniam S. Wnt Pathway Activation Increases Hypoxia Tolerance during Development. PLos One.  2014 Aug 5;9(8):e103292. PMID:25093834.
  7. Scortegagna M, Kim H, Li JL, Yao H, Brill LM, Han J, Lau E, Bowtell D, Haddad GG, Kaufman RJ, Ronai ZA. Fine tuning of the UPR by the ubiquitin ligases Siah1/2. PLoS Genet. 2014 May 8;10(5):e1004348. doi: 10.1371/journal.pgen.1004348. eCollection 2014 May. PMID: 24809345
  8. Gan Z, Wang J, Salomonis N, Stowe JC, Haddad GG, McCulloch AD, Altintas I, Zambon AC. MAAMD: a workflow to standardize meta-analyses and comparison of affymetrix microarray data. 2014.  BMC Bioinformatics. 2014 Mar 12;15:69. PMID:24621103.
  9. Gu XQ, Pamenter ME, Siemen D, Sun X, Haddad GG. Mitochondrial but not plasmalemmal BK channels are hypoxia-sensitive in human glioma. Glia. 2014. 62(4):504-513. PMID:2444624.
  10. Udpa N, Ronen R, Zhou D, Liang J, Stobdan T, Appenzeller O, Yin Y, Du Y, Guo L, Cao R, Wang Y, Jin X, Huang C, Jia W, Cao D, Guo G, Claydon VE, Hainsworth R, Gamboa JL, Zibenigus M, Zenebe G, Xue J, Liu S, Frazer KA, Li Y, Bafna V, Haddad GG. Whole genome sequencing of Ethiopian highlanders reveals conserved hypoxia tolerance genes. Genome Biol. 2014. 15(2):R36  PMID:24555826